Storage and Thawing Practices of Expressed Breast Milk and Their Association with Growth in Infants Aged 0–6 Months
Abstract
Background: Expressed breast milk (EBM) is one of the most commonly used alternatives for breastfeeding among working mothers to ensure that infants continue to receive adequate nutrition. The quality of expressed breast milk is highly influenced by proper storage and thawing practices. Inappropriate handling during these processes may reduce nutritional content and increase the risk of contamination, potentially affecting infant growth.
Methods: This study employed a quantitative cross-sectional design using a total sampling technique involving 391 respondents. Data were analyzed using univariate and bivariate analyses. The Chi-square test was applied to examine the relationships between EBM storage practices, EBM thawing practices, and infant growth.
Results: Statistical analysis revealed a significant association between expressed breast milk storage practices and infant growth (p < 0.001). A significant association was also found between expressed breast milk thawing practices and infant growth (p < 0.001).
Conclusion: Proper storage and thawing practices of expressed breast milk play an important role in supporting the growth of infants aged 0–6 months.
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References
Agustina, I., & Noviasari, I. (2021). Education on how to express and correct breast milk storage techniques for working mothers. Journal of Community Service for Health, 2(2), 047–050. https://doi.org/10.26699/jcsh.v2i2.art.p047-050
BPS Kota Depok. (2024). Depok municipality employment profile: Sakernas results August 2024.https://depokkota.bps.go.id/id/publication/2025/05/27/7ed5d3b4e0db5f79fa66b377/depok-municipality-employment-profile-sakernas-results-august-2024.html
Brockway, M. (Merilee), Daniel, A. I., Reyes, S. M., Granger, M., McDermid, J. M., Chan, D., Refvik, R., Sidhu, K. K., Musse, S., Patel, P. P., Monnin, C., Lotoski, L., Geddes, D., Jehan, F., Kolsteren, P., Allen, L. H., Hampel, D., Eriksen, K. G., Rodriguez, N., & Azad, M. B. (2024). Human milk macronutrients and child growth and body composition in the first two years: A systematic review. Advances in Nutrition, 15(1), 100149. https://doi.org/10.1016/j.advnut.2023.100149
Chen, G., Xu, R., Zhang, J., Yang, M., Fan, J., Huang, Y., & Sun, X. (2024). Composition of breast milk from mothers of premature and full-term infants and its influence in Z-Scores for infant physical growth. BMC Pediatrics, 24(1), 1–8.
Edemba, P. W., Irimu, G., & Musoke, R. (2022). Knowledge attitudes and practice of breastmilk expression and storage among working mothers with infants under six months of age in Kenya. International Breastfeeding Journal, 17(1), 1–8. https://doi.org/10.1186/s13006-022-00469-6
Hernández-Olivas, E., Asensio-Grau, A., Calvo-Lerma, J., Heredia, A., & Andrés, A. (2023). Impact of refrigeration and freezing-thawing of breast milk on in vitro digestibility and liposoluble vitamin bioaccessibility in breast-fed infants. Lwt, 187(September). https://doi.org/10.1016/j.lwt.2023.115348
Kementerian Kesehatan Republik Indonesia. (2022). Riset Kesehatan Dasar (Riskesdas) 2022. https://www.kemkes.go.id/resources/download/general/Hasil Riskesdas 2022.pdf
Kemenkes RI. (2023). Peraturan Presiden Republik Indonesia Nomor 72 Tahun 2021 tentang Percepatan Penurunan Stunting. https://peraturan.bpk.go.id/Details/174964/perpres-no-72-tahun-2021
Laksono, A. D., Wulandari, R. D., Ibad, M., & Kusrini, I. (2021). The effects of mother’s education on achieving exclusive breastfeeding in Indonesia. BMC Public Health, 21(1), 1–6. https://doi.org/10.1186/s12889-020-10018-7
Liang, N., Koh, J., Kim, B. J., Ozturk, G., Barile, D., & Dallas, D. C. (2022). Structural and functional changes of bioactive proteins in donor human milk treated by vat-pasteurization, retort sterilization, ultra-high-temperature sterilization, freeze-thawing and homogenization. Frontiers in Nutrition, 9. https://doi.org/10.3389/fnut.2022.926814
Mercer, R. T. (2004). Clinical scholarship becoming a mother versus maternal role attainment. Journal of Nursing Scholarship, 36(3), 226–232.
Pambudi, W., Dewanto, N. E. F., Yusra, Y., Werdhani, R. A., Endaryanto, A., Fahmida, U., Alatas, F. S., & Syarif, B. H. (2024). Perceptions, practices, and associated factors towards expressed breastfeeding among mothers in Jakarta, Indonesia. Paediatrica Indonesiana(Paediatrica Indonesiana), 64(2), 168–175. https://doi.org/10.14238/pi64.2.2024.168-75
Putri, D. K., Syaidah, R., & Jusuf, A. A. (2024). The effects of different storage conditions on leukocytes in human breast milk. Sultan Qaboos University Medical Journal, 24(1), 91–98. https://doi.org/10.18295/squmj.12.2023.084
Siviroj, P., Ruangsuriya, J., & Ongprasert, K. (2024). Changes in protein levels during the storage and warming of breast milk in a domestic environment. Children, 11(9), 1–10. https://doi.org/10.3390/children11091133
Syahri, I. M., Laksono, A. D., Fitria, M., Rohmah, N., Masruroh, M., & Ipa, M. (2024). Exclusive breastfeeding among Indonesian working mothers: does early initiation of breastfeeding matter? BMC Public Health, 24(1), 1–10. https://doi.org/10.1186/s12889-024-18619-2
Stinson, L. F., George, A., Gridneva, Z., Jin, X., Lai, C. T., & Geddes, D. T. (2024). Effects of different thawing and warming processes on human milk composition. Journal of Nutrition, 154(2), 314–324. https://doi.org/10.1016/j.tjnut.2023.11.027
UNICEF. (2021). Infant and Young Child Feeding: Guideline for Promoting Exclusive Breastfeeding. https://www.unicef.org
World Health Organization. (2020). Global Nutrition Report. https://www.who.int/nutrition/publications/global-report
World Health Organization. (2024). Hand expression and safe storage of breast milk. Geneva: World Health Organization. Retrieved from https://cdn.who.int/media/docs/default-source/mca-documents/nbh/enc-course/revised-resources/supplemental-materials/breast-milk-feeding-alternative-methods/handout-hand-expression-and-safe-storage.pdf
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